Between 2017 and 2018, several farms of Bulgaria reported H5 H5 High Pathogen H5 (HPAI) virus outbreaks. In this study, we used genomic and traditional epidemiological analyzes to trace the origin and subsequent propagation of these epidemics in Bulgaria. The two methods indicate two distinct incursions, a limited to the northeastern region of Dobrich, and another largely limited to Central and Eastern Bulgaria, including places such as Plovdiv, Sliven and Stara Zagora, as well as a virus from the western region of vidin. The two epidemics probably come from different European ancestors 2.3.4.4b virus circulating in 2017. Viruses have probably been introduced by wild birds or commercial poultry links in 2017 and continued to circulate, but because of the lack of sampling. and contemporary sequences of wild bird viruses. In Bulgaria, the precise way and the schedule of the introduction can not be determined.

The analysis of entire genomes indicates a total lack of reallocation in all segments, but the engine of the matrix protein (MP), which has several smaller clusters associated with different European viruses 2.3.4.4b. The ancestral reconstruction of the host states of the hemagglutinin virus gene (ha) of the viruses involved in epidemics suggests that the transmission is driven by domestic ducks in galliform poultry. Thus, according to current evidence, we suggest the surveillance of domestic ducks because they constitute an epidemiological species for a subclinical infection. Monitor the propagation due to the movement between farms in regions and links to poultry production systems in European countries can help predict and prevent future epidemics. Line 2.3.4.4b which caused the circulation of the largest poultry epidemic registered in Europe and the risk of subsequent transmission of wild birds during migration remains

Genetic diversity, reproductive capacity and alkaloids are in three endemic alkanna species

The endemic species Balkans Alkanna Primuliflora Grateb., A. Stribnyi Velen. And A. Graeca wood. & Spruner have limited distribution in the Balkan peninsula and a great variation in morphological characteristics. The populations of the three alkanna species of the Bulgarian flora are small and fragmented. There are no previous reports on the chemical profile or the embryology of these species. The hypothesis was that the limited distribution of A. Primululiflora, A. Stribnyi, and A. Graeca was due to their reproductive capacity and genetic diversity. In addition, we hypothesized that the three species will contain pyrrolizidin (not) alkaloids, such as other alkanna (boraginaceae) species, but they would have a differential composition of the alkaloids.

The genetic structure and differentiation of the population showed a clear distinction between the species and revealed average levels of genetic diversity between the natural populations of the three species of Alkanna. The embryotological survey observed the stability of processes in the male and feminine-generating spheres and the high viability of the mature pollen and the embryo of the three species. The normal training of male and feminine gametophytes without deviations or degenerative processes, and the observed levels of the genetic diversity between people of Alkanna are important to maintain the size and resilience of Alkanna populations. Eight alkaloids were identified by GC-MS in A. Primuliflora and A. Graeca and six alkaloids of A. stribnyi.

The main alkaloids of pyrrolizidine (not) of all species studied were triangularine. A. Primuliflora and A. Graeca showed a similar chemical composition comprising 9-angeloylreginecine, 7-tumitum, 9-tumitable, triangularicine, dihydroxytrangue, dihydroxytriangulin, while in A. stribrnyi 9-tagloylroncine, triangularicine and the dihydroxytriangularicin have not been found. This is the first report on the presence of steps in A. Primuliflora, A. Stribnyi and A. Graeca.

Machine learning approaches for sex estimating using cranial measures

The purpose of this study is to apply support vector machines (SVM) and the artificial neuronal network (ANN) as sex classifiers and generate useful classification templates for a measurement-based sexual estimate. Cranials. In addition, the performance of the generated sub-symbolic automatic learning models is compared to models developed by logistic regression (LR). The study was carried out on images of calculated tomography of 393 Bulgarian adults (169 males and 224 women).

The three-dimensional coordinates of 47 benchmarks were acquired and used to calculate the cranial measures. A total of 64 measures (linear distances, angles, triangle and height areas) and 22 indices were calculated. Two datasets have been assembled, including linear measurements and all measurements and measurements and index, respectively. A third additional dataset comprising all possible intermediate distances between landmarks has been constructed. Two automatic-SVM and ANN automatic learning algorithms and a traditional statistical analysis LR-have been applied to generate sexual estimation models. In addition, two advanced attribute selection techniques (Weka Bestfirst and Weka GeneticSearch) were used. The accuracy of the model classification has been evaluated using a cross-validation procedure of 10 × 10 times. The three methods obtained accuracy results greater than 95%.

Wang Resin
A6776-100000	ApexBio	100 g	EUR 240
Description: Wang Resin

Wang Resin
A6776-25000	ApexBio	25 g	EUR 150
Description: Wang Resin

GST-Sefinose Resin
BSP031	Bio Basic	5ml	EUR 174.84

GST-Sefinose Resin
BSP031-1	Bio Basic	25ml	EUR 498.48

Glutathione Agarose Resin
4B-GLU-10	ABTBeads	10 ml	EUR 207.6

Glutathione Agarose Resin
4B-GLU-100	ABTBeads	100 ml	EUR 1195.2

Biotin Agarose Resin
4BCL-BI-10	ABTBeads	10 ml	EUR 493.2

Biotin Agarose Resin
4BCL-BI-5	ABTBeads	5 ml	EUR 282

Aminomethyl Polystyrene Resin
5-08191	CHI Scientific	25g	Ask for price

Aminomethyl Polystyrene Resin
5-08192	CHI Scientific	100g	Ask for price

Aminomethyl Polystyrene Resin
5-08193	CHI Scientific	25g	Ask for price

Aminomethyl Polystyrene Resin
5-08194	CHI Scientific	100g	Ask for price

MP-EDC resin
20-abx095129	Abbexa	EUR 1262.40 EUR 526.80 EUR 260.40	100 g 25 g 5 g

MP-TEMPO resin
20-abx095130	Abbexa	EUR 1262.40 EUR 526.80 EUR 260.40	100 g 25 g 5 g

MP-Aminomethyl resin
20-abx095132	Abbexa	EUR 393.60 EUR 226.80	100 g 25 g

MP-Acetylacetone resin
20-abx095133	Abbexa	EUR 526.80 EUR 276.00	100 g 25 g

MP-Piperazinomethyl resin
20-abx095134	Abbexa	EUR 526.80 EUR 276.00	100 g 25 g

MP-Diethylenetriamine resin
20-abx095136	Abbexa	EUR 543.60 EUR 276.00	100 g 25 g

MP-Carbonate resin
20-abx095138	Abbexa	EUR 560.40 EUR 292.80	100 g 25 g

MP-Morpholinomethyl resin
20-abx095139	Abbexa	EUR 560.40 EUR 292.80	100 g 25 g

MP-Thiosulfate resin
20-abx095140	Abbexa	EUR 560.40 EUR 292.80	100 g 25 g

MP-Mercaptomethyl resin
abx095141-100g	Abbexa	100 g	EUR 610.8

MP-TMT resin
20-abx095142	Abbexa	EUR 693.60 EUR 326.40	100 g 25 g

Ni-NTA Resin
20-abx098108	Abbexa	EUR 610.80 EUR 292.80	25 ml 5 ml

Ni-IDA Resin
20-abx098109	Abbexa	EUR 543.60 EUR 276.00	25 ml 5 ml

Protein A Resin
abx098111-5ml	Abbexa	5 ml	EUR 477.6

Protein G Resin
abx098112-5ml	Abbexa	5 ml	EUR 526.8

Rink Amide Resin
A6576-100000	ApexBio	100 g	EUR 1136.4
Description: Rink resin is originally developed for SPS of peptide amides. Now the scope of its application is extended from carboxylic amides to the immobilization of amines, substituted amides.

Rink Amide Resin
A6576-25000	ApexBio	25 g	EUR 358.8
Description: Rink resin is originally developed for SPS of peptide amides. Now the scope of its application is extended from carboxylic amides to the immobilization of amines, substituted amides.

DHP HM Resin
A6789-100000	ApexBio	100 g	EUR 616.8
Description: DHP HM Resin

DHP HM Resin
A6789-25000	ApexBio	25 g	EUR 243.6
Description: DHP HM Resin

Protein A Agarose Resin
BSP095-7	Bio Basic	1ml	EUR 112.2

Protein A Agarose Resin
BSP095-8	Bio Basic	5ml	EUR 294.9

Protein A Sefinose Resin
BSP096-25	Bio Basic	12.5ml	EUR 843

Protein A Sefinose Resin
BSP096-5	Bio Basic	2.5ml	EUR 232.26

Nickel NTA Agarose Resin
6BCL-NTANi-100	ABTBeads	100 ml	EUR 781.2

Nickel NTA Agarose Resin
6BCL-NTANi-25	ABTBeads	25 ml	EUR 260.4

Nickel NTA Agarose Resin
6BCL-NTANi-500	ABTBeads	500 ml	EUR 3100.8

Protein L Agarose Resin
4BCLPL-10	ABTBeads	10 ml	EUR 471.6

Protein L Agarose Resin
4BCLPL-2	ABTBeads	2 ml	EUR 236.4

Protein L Agarose Resin
4BCLPL-5	ABTBeads	5 ml	EUR 340.8

Fmoc-Ala-Wang resin
5-07835	CHI Scientific	5g	Ask for price

Fmoc-Ala-Wang resin
5-07836	CHI Scientific	25g	Ask for price

Fmoc-Acp-Wang resin
5-07841	CHI Scientific	5g	Ask for price

Fmoc-Acp-Wang resin
5-07842	CHI Scientific	25g	Ask for price

Fmoc-Cha-Wang Resin
5-07863	CHI Scientific	5g	Ask for price

Fmoc-Cha-Wang Resin
5-07864	CHI Scientific	25g	Ask for price

Fmoc-Cit-Wang resin
5-07867	CHI Scientific	5g	Ask for price

Fmoc-Cit-Wang resin
5-07868	CHI Scientific	25g	Ask for price

Fmoc-Gly-Wang resin
5-07891	CHI Scientific	5g	Ask for price

Fmoc-Gly-Wang resin
5-07892	CHI Scientific	25g	Ask for price

Fmoc-Gly-Wang resin
5-07893	CHI Scientific	5g	Ask for price

Fmoc-Gly-Wang resin
5-07894	CHI Scientific	25g	Ask for price

Fmoc-Ile-Wang resin
5-07899	CHI Scientific	5g	Ask for price

Fmoc-Ile-Wang resin
5-07900	CHI Scientific	25g	Ask for price

Fmoc-Leu-Wang resin
5-07903	CHI Scientific	5g	Ask for price

Fmoc-Leu-Wang resin
5-07904	CHI Scientific	25g	Ask for price

Fmoc-Leu-Wang resin
5-07905	CHI Scientific	5g	Ask for price

Fmoc-Leu-Wang resin
5-07906	CHI Scientific	25g	Ask for price

Fmoc-Lys-Wang resin
5-07907	CHI Scientific	5g	Ask for price

Fmoc-Lys-Wang resin
5-07908	CHI Scientific	25g	Ask for price

Fmoc-Met-Wang resin
5-07923	CHI Scientific	5g	Ask for price

Fmoc-Met-Wang resin
5-07924	CHI Scientific	25g	Ask for price

Fmoc-Phe-Wang resin
5-07929	CHI Scientific	5g	Ask for price

Fmoc-Phe-Wang resin
5-07930	CHI Scientific	25g	Ask for price

Fmoc-Phe-Wang resin
5-07931	CHI Scientific	5g	Ask for price

Fmoc-Phe-Wang resin
5-07932	CHI Scientific	25g	Ask for price

Fmoc-Pro-Wang resin
5-07947	CHI Scientific	5g	Ask for price

Fmoc-Pro-Wang resin
5-07948	CHI Scientific	25g	Ask for price

Fmoc-Pro-Wang resin
5-07949	CHI Scientific	5g	Ask for price

Fmoc-Pro-Wang resin
5-07950	CHI Scientific	25g	Ask for price

Fmoc-Tle-Wang Resin
5-07965	CHI Scientific	5g	Ask for price

Fmoc-Tle-Wang Resin
5-07966	CHI Scientific	25g	Ask for price

Fmoc-Thr-Wang resin
5-07967	CHI Scientific	5g	Ask for price

Fmoc-Thr-Wang resin
5-07968	CHI Scientific	25g	Ask for price

Fmoc-Trp-Wang resin
5-07975	CHI Scientific	5g	Ask for price

Fmoc-Trp-Wang resin
5-07976	CHI Scientific	25g	Ask for price

Fmoc-Val-Wang resin
5-07989	CHI Scientific	5g	Ask for price

Fmoc-Val-Wang resin
5-07990	CHI Scientific	25g	Ask for price

2-Chlorotrityl Chloride Resin
5-08183	CHI Scientific	25g	Ask for price

2-Chlorotrityl Chloride Resin
5-08184	CHI Scientific	100g	Ask for price

2-Chlorotrityl Chloride Resin
5-08185	CHI Scientific	25g	Ask for price

2-Chlorotrityl Chloride Resin
5-08186	CHI Scientific	100g	Ask for price

Fmoc-Leu-Tcp Resin
5-08289	CHI Scientific	5g	Ask for price

Fmoc-Leu-Tcp Resin
5-08290	CHI Scientific	25g	Ask for price

Protein A-Agarose Resin
55005	AAT Bioquest	5 mL	EUR 367.2

Fmoc-Gly-Wang resin
20-abx095122	Abbexa	EUR 661.20 EUR 309.60	100 g 25 g

MP-Sulfonic acid resin
20-abx095135	Abbexa	EUR 526.80 EUR 276.00	100 g 25 g

2-Chlorotrityl Chloride resin
20-abx095137	Abbexa	EUR 560.40 EUR 1078.80 EUR 276.00 EUR 1930.80	100 g 250 g 25 g 500 g

Rink Amide AM resin
20-abx095146	Abbexa	EUR 961.20 EUR 4987.20 EUR 1746.00 EUR 393.60 EUR 2698.80	100 g 1 kg 250 g 25 g 500 g

Rink Amide-AM Resin
A6780-100000	ApexBio	100 g	EUR 872.4
Description: Rink Amide-AM Resin

Rink Amide-AM Resin
A6780-25000	ApexBio	25 g	EUR 466.8
Description: Rink Amide-AM Resin

Rink Amide-AM Resin
A6780-5000	ApexBio	5 g	EUR 262.8
Description: Rink Amide-AM Resin

2-Chlorotrityl Chloride Resin
A6791-100000	ApexBio	100 g	EUR 393.6
Description: 2-Chlorotrityl Chloride Resin

2-Chlorotrityl Chloride Resin
A6791-25000	ApexBio	25 g	EUR 170.4
Description: 2-Chlorotrityl Chloride Resin

Fmoc-Phe-Wang resin
A6831-25000	ApexBio	25 g	EUR 246
Description: Fmoc-Phe-Wang resin

Fmoc-Phe-Wang resin
A6831-5000	ApexBio	5 g	EUR 104.4
Description: Fmoc-Phe-Wang resin

Fmoc-Ile-Wang resin
A6836-25000	ApexBio	25 g	EUR 246
Description: Fmoc-Ile-Wang resin

Fmoc-Ile-Wang resin
A6836-5000	ApexBio	5 g	EUR 104.4
Description: Fmoc-Ile-Wang resin

Fmoc-Gly-Wang resin
A6839-25000	ApexBio	25 g	EUR 246
Description: Fmoc-Gly-Wang resin

Fmoc-Gly-Wang resin
A6839-5000	ApexBio	5 g	EUR 104.4
Description: Fmoc-Gly-Wang resin

Protein A Agarose Resin
PA09-100	ABTBeads	100 ml	EUR 2512.8

Protein A Agarose Resin
PA09-25	ABTBeads	25 ml	EUR 1110

Protein A Agarose Resin
PA09-5	ABTBeads	5 ml	EUR 349.2

Protein G Agarose Resin
SF022-PG	Bio Basic	5ml	EUR 930

Streptavidin 6HC Agarose Resin
STV6HC-10	ABTBeads	10 ml	EUR 478.8

Streptavidin 6HC Agarose Resin
STV6HC-20	ABTBeads	20 ml	EUR 822

Streptavidin 6HC Agarose Resin
STV6HC-5	ABTBeads	5 ml	EUR 284.4

AFS CARTRIDGE ALL RESIN
CP1ALLRES	Scientific Laboratory Supplies	EACH	EUR 500.46

MabSelect PrismA Resin - 25mL
17549801	Scientific Laboratory Supplies	EACH	EUR 2714.34

MabSelect PrismA Resin - 200mL
17549802	Scientific Laboratory Supplies	EACH	EUR 7498.92

Resin cartridges for XD250
WAT2102	Scientific Laboratory Supplies	PK2	EUR 314.64

Purified GFP
AG45-0205-Z	Immunology Consultants Laboratory	0.1 mg	EUR 273.6

Ovalbumin, purified
O705084	Bio Basic	1g	EUR 80.88

Purified Water
H2O1	Scientific Laboratory Supplies	1L	EUR 15.96

Purified Water
H2O10	Scientific Laboratory Supplies	10L	EUR 78.66

Purified Water
H2O25	Scientific Laboratory Supplies	25L	EUR 69.54

Purified Water
H2O5	Scientific Laboratory Supplies	5L	EUR 22.8

Bromoacetyl resin (200-400 mesh)
D-2555.0001	Bachem	1.0g	EUR 166.8

Bromoacetyl resin (200-400 mesh)
D-2555.0005	Bachem	5.0g	EUR 529.2

Fmoc-1-Nal-Wang resin
5-07831	CHI Scientific	5g	Ask for price

Fmoc-1-Nal-Wang resin
5-07832	CHI Scientific	25g	Ask for price

Fmoc-2-Nal-Wang resin
5-07833	CHI Scientific	5g	Ask for price

Fmoc-2-Nal-Wang resin
5-07834	CHI Scientific	25g	Ask for price

Fmoc-D-Ala-Wang resin
5-07837	CHI Scientific	5g	Ask for price

Fmoc-D-Ala-Wang resin
5-07838	CHI Scientific	25g	Ask for price

Fmoc-Arg(Pbf)-Wang resin
5-07843	CHI Scientific	5g	Ask for price

Fmoc-Arg(Pbf)-Wang resin
5-07844	CHI Scientific	25g	Ask for price

Fmoc-Asn(Trt)-Wang resin
5-07847	CHI Scientific	5g	Ask for price

Fmoc-Asn(Trt)-Wang resin
5-07848	CHI Scientific	25g	Ask for price

Fmoc-Asp(OtBu)-Wang resin
5-07853	CHI Scientific	5g	Ask for price

Fmoc-Asp(OtBu)-Wang resin
5-07854	CHI Scientific	25g	Ask for price

Fmoc-Asp-OAll-Wang Resin
5-07855	CHI Scientific	5g	Ask for price

Fmoc-Asp-OAll-Wang Resin
5-07856	CHI Scientific	25g	Ask for price

Fmoc-Cys(Acm)-Wang resin
5-07869	CHI Scientific	5g	Ask for price

Fmoc-Cys(Acm)-Wang resin
5-07870	CHI Scientific	25g	Ask for price

Fmoc-Cys(Trt)-Wang resin
5-07871	CHI Scientific	5g	Ask for price

Fmoc-Cys(Trt)-Wang resin
5-07872	CHI Scientific	25g	Ask for price

Fmoc-Cys(Trt)-Wang resin
5-07873	CHI Scientific	5g	Ask for price

Fmoc-Cys(Trt)-Wang resin
5-07874	CHI Scientific	25g	Ask for price

Fmoc-Gln(Trt)-Wang resin
5-07879	CHI Scientific	5g	Ask for price

Fmoc-Gln(Trt)-Wang resin
5-07880	CHI Scientific	25g	Ask for price

Fmoc-Glu(OAll)-Wang resin
5-07883	CHI Scientific	5g	Ask for price

The best precision (96.1 ± 0.5%) was obtained by SVM and it was statistically higher than the best results obtained by ANN and LR. SVM and ANN have reached a higher precision by training on complete data sets that selection datasets, with the exception of the sample described by the intermediate distances, where the reduction of the attributes by the algorithm of Genecesearch has improved accuracy. The purpose of this study is to deepen our knowledge of painting materials and technological characteristics of iconography during the Bulgarian national renaissance. Our efforts focused on the study of the murals of the Catholicon Church (Main Church) “The Nativity of the Virgin” of the Rila Monastery, which is one of Bulgarian historical monuments. A series of micro-samples from the altar wall paints were analyzed by a combination of totenuated reflectance of the totenuated reflectance transform infrared (ATR-FTIIR), micro-Raman spectroscopy, X-ray spectroscopy with scanning electron microscopy (SEM-EDS), X-Removal Diffraction (XRD), Thermogravimetric Analysis (TGA) and Optical Microscopy (OM).