Checklist and provisional atlas of singing cicadas (Hemiptera: Cicadidae) of Bulgaria, based on bioacoustics

Background: The Cicadas Singers (hemiptera: cigadidae) of Bulgaria were poorly known. There are records published for 14 species: Cicada Orni, Lyrists Plebejus, Cicadatra Atra, Cicadatra Hyalina, Cicadatra Persica, Cicadatta Persica, Cicadetta Montana, Cicadetta Mediterranea, Timissaleta Dimissa, Oliglena Tibialis, Tympanistalis Gastrica, Pagiphora Annulata, Saiticula Coriaria, Tibicina Haematodes and Tibicina Steveni.

New information: Two species of this list were doubtful at the beginning of our study, because Tympanistalna Gastrica is distributed in the center and southern Portugal and Saiala Coriaria is a North African species. We have verified three main institutional collections hosted in Sofia, Bulgaria: the National Museum of Natural History (SOFM), the Institute of Zoology (ZISB) and the Faculty of Biology at Sofia University “St. Kliment ohridski »(BFU). We have confirmed 11 species mentioned in the literature, with the exception of Cicadetta Mediterranea and found two additional species: Cicadata Plassyptera and Cicadetta Macedonica (BFU specimens were confirmed bioacoustically).

Based on this knowledge, we still studied the Bulgaria’s singing cicadas with the use of morphological and bioacoustic methods in the years 2008, 2009, 2010, 2012, 2012, 2016 and 2019. We could not confirm the presence of Cicadata Persica and Cicadetta Mediterranea, but has Found three additional species: Cicadetta BreviNnis s. Lat., Cicadetta Cantilatrix and Tettettula Pygmea. Using bioacoustic methods, we also detected unknown song models, which could belong to three or four additional taxa, which must be described. The Bulgarian fauna of Cicada singing at the same time is composed of 16 potential species confirmed and 3-4.

 

SCN1A mutation spectrum in a cohort of Bulgarian patients at the GEFS + phenotype

BACKGROUND: Dravet syndrome (DS) is the most serious form of widespread epilepsy with febrile crisis syndrome and febrile supports (GEFS +) with a clear genetic component in 85% of cases. It is characterized by a seizure caused by fever of about six months and a deterioration of subsequent development later in life.

Methods: In the current study, 60 patients with crises and suspicions caused by fever, either FEMS + (50 patients) or DS (10 patients) were referred for the analysis of the SCN1A genes sequence.

Results: SCN1A Gene Sequencing revealed clinically significant variants in 11 patients (18.3%); Seven pathogens (11.7%) and four probable pathogens (6.7%). Five of these variants were not reported previously. Among the preselected group of ten DS patients, five had SCN1A pathogenic variants that confirmed the diagnosis of DS. In four patients with preliminary diagnosis GEFS +, the detected variant SCN1A allowed us to specify the diagnosis of DS in these patients. Thus, the SCN1A sequencing resulted in a confirmation of the 50% (5/10) genetic diagnosis of DS patients, as well as to clarify the diagnosis of DS in 8% of FEMS + patients (450). In this study, four patients with truncating mutations have had refractory crises and additional psychomotor anomalies. In addition, Missense pathogenic mutations have been detected in three children with comparable phenotypes, which favors observations that mutations despite critical channel function regions can cause a devastating epileptic disease.

Conclusions: This is the first systematic screening of the SCN1A gene in our country, which expands the spectrum of SCN1A variants with five new variants in Bulgaria and demonstrates the clinical utility of CNN1A confirmatory tests, which helps clinicians make Early and precise diagnoses. It is important for better monitoring, the choice of appropriate treatment, the prevention of the development of refractory seizures and neuropsychological complications. Identification of pathogenic variants in the SCN1A in the milder cases of FEMS + and serious DS, will help provide adequate prenatal diagnosis and improve genetic tips for affected families.

 Checklist and provisional atlas of singing cicadas (Hemiptera: Cicadidae) of Bulgaria, based on bioacoustics
Checklist and provisional atlas of singing cicadas (Hemiptera: Cicadidae) of Bulgaria, based on bioacoustics

Respiratory symptoms of exposure to workplace substances among Bulgarian dentists

Objectives: Dentists are exposed to a variety of airborne chemicals that can serve as irritants and sensitizers and can give rise to work-related respiratory symptoms. The objective of this study was to estimate the prevalence of respiratory symptoms of exposure to workplace substances and risk factors involved in Bulgarian dentists.

Methods: A cross-sectional study was conducted among Bulgarian dentists using a self-recall questionnaire. A direct acyclic chart (DAG) has been developed to illustrate direct and indirect causal paths between exposure to irritants and / or allergens from the dental environment and work-related respiratory symptoms in dentists. A multiple logistic regression analysis was performed to study the relationship between sex, work experience, daily exposure to chemicals from the dental environment, a history of atopic disorders and respiratory symptoms related to the job.

Results: A total of 4675 dentists completed the questionnaire (response rate 48.1%). The prevalence of self-reported respiratory symptoms was 20.7%. The most commonly repeated causes of respiratory reactions were disinfectants (65.7%) and materials based on acrylic resins (29.7%). Factors associated with work-related respiratory symptoms are a personal history of confidence interval of asthma, work experience> 20 years and female sex.

 

Cap Phenolic GPI 18-415 Screw Cap Teflon Liner Corning

TIS1560 PK192
EUR 142.8

Black Phenolic screw thread cap open top linerless autoclavable

VIA2522 PK144
EUR 60

Reusable Phenolic GPI 13-415 Threaded Screw Cap with Teflon Liner Corning

TIS1556 PK288
EUR 321.6

CORNING® REUSABLE PHENOLIC GPI 18-415 THREADED SCREW CAP WITH RUBBER LINER

9999-182 32/pk
EUR 99.6
Description: General Apparatus; Stoppers

CORNING® REUSABLE PHENOLIC GPI 24-410 THREADED SCREW CAP WITH RUBBER LINER

9999-241 36/pk
EUR 118.8
Description: General Apparatus; Stoppers

CORNING® REUSABLE PHENOLIC GPI 28-400 THREADED SCREW CAP WITH RUBBER LINER

9999-28 144/pk
EUR 288
Description: General Apparatus; Stoppers

CORNING® REUSABLE PHENOLIC GPI 28-410 THREADED SCREW CAP WITH RUBBER LINER

9999-281 12/pk
EUR 61.2
Description: General Apparatus; Stoppers

CORNING® REUSABLE PHENOLIC GPI 38-400 THREADED SCREW CAP WITH RUBBER LINER

9999-38 36/pk
EUR 266.4
Description: General Apparatus; Stoppers

CORNING® REUSABLE PHENOLIC GPI 40-400 THREADED SCREW CAP WITH RUBBER LINER

9999-40 72/pk
EUR 168
Description: General Apparatus; Stoppers

Disposable Phenolic GPI 13-415 Threaded Screw Cap with Rubber Liners Corning

TIS1550 PK1000
EUR 349.2

Disposable Phenolic GPI 18-415 Threaded Screw Cap with Rubber Liners Corning

TIS1554 PK1000
EUR 488.4

Corning 25cm2 Rectangular Canted Neck Cell Culture Flask with Phenolic-Style Cap

430372 PK500
EUR 746.4

Corning 175cm2 Rectangular Angled Neck Cell Culture Flask with Phenolic-Style Cap

431085 PK50
EUR 380.4

Phenolics Mix B

8040-B 1ML
EUR 74.4

Phenolics Mix

CLPS-A 1ML
EUR 52.8

Phenolics Mix A

CLPS-AI 1ML
EUR 112.8

4-(2',4'-Dimethoxyphenyl-Fmoc-aminomethyl)-phenoxymethyl-polystyrene resin (200-400 mesh, 0.80-1.20 mmol/g)

D-2080.0001 1.0g
EUR 151.2

4-(2',4'-Dimethoxyphenyl-Fmoc-aminomethyl)-phenoxymethyl-polystyrene resin (200-400 mesh, 0.80-1.20 mmol/g)

D-2080.0005 5.0g
EUR 471.6

4-(2',4'-Dimethoxyphenyl-Fmoc-aminomethyl)-phenoxymethyl-polystyrene resin (200-400 mesh, 0.80-1.20 mmol/g)

D-2080.0025 25.0g
EUR 1748.4

c6030 kit epoxy ec/tds/sal/res

C6030KE ea
EUR 1095.6

c3010 kit epoxy ec/tds/sal/res

C3010KE ea
EUR 910.8

c3030 kit epoxy ec/tds/sal/res

C3030KE ea
EUR 1256.4

Hettich Lid For Rotor Phenol Resistant

CEN0811 EACH
EUR 342

MagSi-S Epoxy 600

MD16010 2 mL
EUR 153.6

MagSi-S Epoxy 600

MD18010 10 mL
EUR 572.4

MagSi-S Epoxy 600

MD19010 100 mL
EUR 3438

c6010 kit epoxy ph/ec/tds/sal/res

C6010PKE ea
EUR 919.2

c6030 kit epoxy ph/ec/tds/sal/res

C6030PKE ea
EUR 1203.6

c3010 kit epoxy ph/ec/tds/sal/res

C3010PKE ea
EUR 1018.8

c3030 kit epoxy ph/ec/tds/sal/res

C3030PKE ea
EUR 1363.2

Epoxy Costuslactone

TBW00951 20mg Ask for price

c6010 kit epoxy ph/ec/tds/sal/res/o2

C6010TE ea
EUR 1167.6

c6030 kit epoxy ph/ec/tds/sal/res/o2

C6030TE ea
EUR 1452

c3010 kit epoxy ph/ec/tds/sal/res/o2

C3010TE ea
EUR 1267.2

c3030 kit epoxy ph/ec/tds/sal/res/o2

C3030TE ea
EUR 1611.6

c3050 kit epoxy ph/ec/sal/tds/res/orp

C3050TE ea
EUR 1935.6

c6010 kit epoxy ph/ec/tds/sal/res/o2/orp

C6010TOE ea
EUR 1317.6

c6010 kit epoxy ec

C6010KE ea
EUR 812.4

c6010 kit epoxy ph

C6010PE ea
EUR 795.6

c6030 kit epoxy ph

C6030PE ea
EUR 1080

c3010 kit epoxy ph

C3010PE ea
EUR 895.2

c3030 kit epoxy ph

C3030PE ea
EUR 1239.6

MagSi-S Epoxy 1.0

MD01010 2 mL
EUR 139.2

MagSi-S Epoxy 1.0

MD03010 10 mL
EUR 483.6

MagSi-S Epoxy 1.0

MD04010 100 mL
EUR 2898

MagSi-S Epoxy 3.0

MD41010 2 mL
EUR 139.2

MagSi-S Epoxy 3.0

MD43010 10 mL
EUR 483.6

MagSi-S Epoxy 3.0

MD44010 100 mL
EUR 2898

Epoxy Magnetic Particles

EM-05-10 10 mL
EUR 267.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Epoxy Magnetic Particles

EM-20-10 10 mL
EUR 267.6
Description: Epoxy Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Epoxy Magnetic Particles

EM-20-100 100 mL
EUR 1716
Description: Epoxy Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

Bottle carrier epoxy small

BGS040 EACH
EUR 121.2

92 L Cooled Incubator epoxy

INC2156 EACH
EUR 3954.66

SLS Electrode pH Epoxy Body BNC

PHE1000 EACH
EUR 78.34

Epoxy-activated Sepharose 6B 15g

17048001 EACH
EUR 760.8

Phenol Std Phenol 2000ug/mL in MeOH

REPHE110 1ML
EUR 110.98

Phenol Std Phenol 100ug/mL in DCM

REPHE119 1ML
EUR 54.05

DiagNano Epoxy Silica Particles, 300 nm

DNG-F062 100 mg
EUR 682.8

DiagNano Epoxy Silica Particles, 500 nm

DNG-F063 100 mg
EUR 682.8

DiagNano Epoxy Silica Particles, 1 μm

DNG-F064 100 mg
EUR 702

DiagNano Epoxy Silica Particles, 1.5 μm

DNG-F065 100 mg
EUR 721.2

DiagNano Epoxy Silica Particles, 3 μm

DNG-F066 100 mg
EUR 682.8

DiagNano Epoxy Silica Particles, 4 μm

DNG-F067 100 mg
EUR 682.8

DiagNano Epoxy Silica Particles, 5 μm

DNG-F068 100 mg
EUR 682.8

DiagNano Epoxy Silica Particles, 10 μm

DNG-F069 100 mg
EUR 721.2

DiagNano Epoxy Silica Particles, 15 μm

DNG-F070 100 mg
EUR 721.2

DiagNano Epoxy Silica Particles, 20 μm

DNG-F071 100 mg
EUR 721.2

Combination pH electrode with epoxy body

9156BNWP EACH
EUR 244.8

DiagPoly Epoxy Polystyrene Particles, 30 μm

DNM-M001 10 mL
EUR 925.2

AM resin

20-abx095149
  • EUR 376.80
  • EUR 710.40
  • EUR 1062.00
  • 100 g
  • 250 g
  • 500 g

ROSS Ultra pH/ATC Triode with epoxy body

8157BNUMD EACH
EUR 553.2

Dumont ESD Epoxy-Coated Tweezers Style 5

M-0302-5-CO 12 EACH
EUR 33
Description: Dumont ESD Epoxy-Coated Tweezers Style 5

Wang Resin

A6776-100000 100 g
EUR 240
Description: Wang Resin

Wang Resin

A6776-25000 25 g
EUR 150
Description: Wang Resin

MBHA resin

20-abx095143
  • EUR 594.00
  • EUR 2766.00
  • EUR 1062.00
  • EUR 292.80
  • EUR 1796.40
  • 100 g
  • 1 kg
  • 250 g
  • 25 g
  • 500 g

Wang resin

20-abx095148
  • EUR 376.80
  • EUR 627.60
  • EUR 1062.00
  • 100 g
  • 250 g
  • 500 g

Epoxy Coated Wire Tube Rack 13mm 6x12 Blue

RAC0012 EACH
EUR 53.88

SLS Slides Diagnostic Epoxy Blk 3 Well 14mm

MIC3404 PK100
EUR 150.14

2-Carboxymethyl-3-preny-2,3-epoxy-1,4-naphthoquinone

TBZ0135 unit Ask for price

Absolute Mag Epoxy Magnetic Particles, 0.5-1.0 μm

WHM-Q075 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, 1.0-2.0 μm

WHM-Q076 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, 2.0-3.0 μm

WHM-Q077 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, 3.0-4.0 μm

WHM-Q078 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, 4.0-5.0 μm

WHM-Q079 10 mL
EUR 670.8

Merrifield resin

20-abx095147
  • EUR 260.40
  • EUR 427.20
  • EUR 627.60
  • 100 g
  • 250 g
  • 500 g

DHP HM Resin

A6789-100000 100 g
EUR 616.8
Description: DHP HM Resin

DHP HM Resin

A6789-25000 25 g
EUR 243.6
Description: DHP HM Resin

SLS Slides Diagnostic Epoxy Blk 10 Well 6-7mm

MIC3414 PK100
EUR 141

Epoxy Coated Wire Urine Cont. Rack 58mm 6x4 Blue

RAC0016 EACH
EUR 87.6

Absolute Mag Epoxy Silica Magnetic Particles, 1.5 µm

SMP-UM05 500 mg
EUR 976.8

Absolute Mag Epoxy Silica Magnetic Particles, 6 µm

SMP-UM18 500 mg
EUR 864

Ni-NTA Resin

20-abx098108
  • EUR 610.80
  • EUR 292.80
  • 25 ml
  • 5 ml

Ni-IDA Resin

20-abx098109
  • EUR 543.60
  • EUR 276.00
  • 25 ml
  • 5 ml

MP-EDC resin

20-abx095129
  • EUR 1262.40
  • EUR 526.80
  • EUR 260.40
  • 100 g
  • 25 g
  • 5 g

MP-TMT resin

20-abx095142
  • EUR 693.60
  • EUR 326.40
  • 100 g
  • 25 g

pH/ATC Triode with epoxy body low maintenance gel

9107BNMD EACH
EUR 306

Phenol

20-abx184753
  • EUR 292.80
  • EUR 260.40
  • 1 kg
  • 500 g

Phenol, 99%

GK6990-100G 100 g
EUR 52.8

Phenol, 99%

GK6990-1KG 1 kg
EUR 93.6

Phenol, 99%

GK6990-500G 500 g
EUR 67.2

Phenol

S-3030 1ML
EUR 44.4

Scienceware centrifuge tube rack epoxy-coated steel

R8384-1EA EACH
EUR 81.6

Scienceware centrifuge tube rack epoxy-coated steel

R8509-1EA EACH
EUR 78

Scienceware centrifuge tube rack epoxy-coated steel

R8634-1EA EACH
EUR 56.4

ROSS Ultra combination pH electrode with epoxy body

8156BNUWP EACH
EUR 481.2

Sure-Flow combination pH electrode with epoxy body

9165BNWP EACH
EUR 363.6

Phenol Red

GT9844-100G 100 g
EUR 132

Phenol Red

GT9844-250G 250 g
EUR 217.2

Phenol Red

GT9844-25G 25 g
EUR 74.4

Phenol Red

GT9844-5G 5 g
EUR 55.2

Phenol red

PB0420 25g
EUR 85.06

Rink Amide Resin

A6576-100000 100 g
EUR 1136.4
Description: Rink resin is originally developed for SPS of peptide amides. Now the scope of its application is extended from carboxylic amides to the immobilization of amines, substituted amides.

Rink Amide Resin

A6576-25000 25 g
EUR 358.8
Description: Rink resin is originally developed for SPS of peptide amides. Now the scope of its application is extended from carboxylic amides to the immobilization of amines, substituted amides.

Protein A Resin

abx098111-5ml 5 ml
EUR 477.6

Protein G Resin

abx098112-5ml 5 ml
EUR 526.8

MP-TEMPO resin

20-abx095130
  • EUR 1262.40
  • EUR 526.80
  • EUR 260.40
  • 100 g
  • 25 g
  • 5 g

Bromoacetyl resin (200-400 mesh)

D-2555.0001 1.0g
EUR 166.8

Bromoacetyl resin (200-400 mesh)

D-2555.0005 5.0g
EUR 529.2

MabSelect PrismA Resin - 200mL

17549802 EACH
EUR 8683.2

11α,12α-Epoxy-3β,23-dihydroxy- 30-norolean-20(29)-en-28,13β-olide

TTE1308 unit Ask for price

HBS w/Phenol Red, 500 ml

408 500 ml
EUR 86.4

Phenol-d6

S-3035 1ML
EUR 44.4

Nonyl Phenol

AT037 1mg
EUR 1641.6

Water Phenol

abx082109-250ml 250 ml
EUR 226.8

Nonyl Phenol

AG037 1 mg
EUR 627.6

USP Sol. Phenol Red TS

USP4101 100ML
EUR 69.2

USP Sol. Phenol Red TS

USP4105 500ML
EUR 61.1

Biotin Agarose Resin

4BCL-BI-10 10 ml
EUR 493.2

Biotin Agarose Resin

4BCL-BI-5 5 ml
EUR 282

GST-Sefinose Resin

BSP031 5ml
EUR 174.84

GST-Sefinose Resin

BSP031-1 25ml
EUR 498.48

MP-Carbonate resin

20-abx095138
  • EUR 560.40
  • EUR 292.80
  • 100 g
  • 25 g

8,​9-​Epoxy-​3-​isobutyryloxy-​10-​(2-​methylbutanoyl)​thymol

HY-N6844 1mg
EUR 268.8

Absolute Mag Epoxy Magnetic Particles, UF Matrix, 1-2 μm

WHM-Q051 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, UF Matrix, 2-3 μm

WHM-Q052 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, UF Matrix, 3-4 μm

WHM-Q053 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, UF Matrix, 4-5 μm

WHM-Q054 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, UF Matrix, 5-7 μm

WHM-Q055 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, γ-Fe2O3, 0.1-1 μm

WHM-Q116 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, γ-Fe2O3, 1-2 μm

WHM-Q117 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, γ-Fe2O3, 2-3 μm

WHM-Q118 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, γ-Fe2O3, 3-4 μm

WHM-Q119 10 mL
EUR 670.8

Absolute Mag Epoxy Magnetic Particles, γ-Fe2O3, 4-5 μm

WHM-Q120 10 mL
EUR 670.8

MP-Aminomethyl resin

20-abx095132
  • EUR 393.60
  • EUR 226.80
  • 100 g
  • 25 g

MP-Thiosulfate resin

20-abx095140
  • EUR 560.40
  • EUR 292.80
  • 100 g
  • 25 g

ROSS combination pH electrode with epoxy body flat surface

8135BN EACH
EUR 469.2

ROSS combination pH electrode with epoxy body flat surface

8135SC EACH
EUR 427.2

Conclusion: Work-related respiratory symptoms are common among dentists and indicate efforts to establish effective programs and techniques for reducing or eliminating direct exposure to chemicals in the air in the dental environment.

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