Checklist and provisional atlas of singing cicadas (Hemiptera: Cicadidae) of Bulgaria, based on bioacoustics

Background: The Cicadas Singers (hemiptera: cigadidae) of Bulgaria were poorly known. There are records published for 14 species: Cicada Orni, Lyrists Plebejus, Cicadatra Atra, Cicadatra Hyalina, Cicadatra Persica, Cicadatta Persica, Cicadetta Montana, Cicadetta Mediterranea, Timissaleta Dimissa, Oliglena Tibialis, Tympanistalis Gastrica, Pagiphora Annulata, Saiticula Coriaria, Tibicina Haematodes and Tibicina Steveni.

New information: Two species of this list were doubtful at the beginning of our study, because Tympanistalna Gastrica is distributed in the center and southern Portugal and Saiala Coriaria is a North African species. We have verified three main institutional collections hosted in Sofia, Bulgaria: the National Museum of Natural History (SOFM), the Institute of Zoology (ZISB) and the Faculty of Biology at Sofia University “St. Kliment ohridski »(BFU). We have confirmed 11 species mentioned in the literature, with the exception of Cicadetta Mediterranea and found two additional species: Cicadata Plassyptera and Cicadetta Macedonica (BFU specimens were confirmed bioacoustically).

Based on this knowledge, we still studied the Bulgaria’s singing cicadas with the use of morphological and bioacoustic methods in the years 2008, 2009, 2010, 2012, 2012, 2016 and 2019. We could not confirm the presence of Cicadata Persica and Cicadetta Mediterranea, but has Found three additional species: Cicadetta BreviNnis s. Lat., Cicadetta Cantilatrix and Tettettula Pygmea. Using bioacoustic methods, we also detected unknown song models, which could belong to three or four additional taxa, which must be described. The Bulgarian fauna of Cicada singing at the same time is composed of 16 potential species confirmed and 3-4.

 

SCN1A mutation spectrum in a cohort of Bulgarian patients at the GEFS + phenotype

BACKGROUND: Dravet syndrome (DS) is the most serious form of widespread epilepsy with febrile crisis syndrome and febrile supports (GEFS +) with a clear genetic component in 85% of cases. It is characterized by a seizure caused by fever of about six months and a deterioration of subsequent development later in life.

Methods: In the current study, 60 patients with crises and suspicions caused by fever, either FEMS + (50 patients) or DS (10 patients) were referred for the analysis of the SCN1A genes sequence.

Results: SCN1A Gene Sequencing revealed clinically significant variants in 11 patients (18.3%); Seven pathogens (11.7%) and four probable pathogens (6.7%). Five of these variants were not reported previously. Among the preselected group of ten DS patients, five had SCN1A pathogenic variants that confirmed the diagnosis of DS. In four patients with preliminary diagnosis GEFS +, the detected variant SCN1A allowed us to specify the diagnosis of DS in these patients. Thus, the SCN1A sequencing resulted in a confirmation of the 50% (5/10) genetic diagnosis of DS patients, as well as to clarify the diagnosis of DS in 8% of FEMS + patients (450). In this study, four patients with truncating mutations have had refractory crises and additional psychomotor anomalies. In addition, Missense pathogenic mutations have been detected in three children with comparable phenotypes, which favors observations that mutations despite critical channel function regions can cause a devastating epileptic disease.

Conclusions: This is the first systematic screening of the SCN1A gene in our country, which expands the spectrum of SCN1A variants with five new variants in Bulgaria and demonstrates the clinical utility of CNN1A confirmatory tests, which helps clinicians make Early and precise diagnoses. It is important for better monitoring, the choice of appropriate treatment, the prevention of the development of refractory seizures and neuropsychological complications. Identification of pathogenic variants in the SCN1A in the milder cases of FEMS + and serious DS, will help provide adequate prenatal diagnosis and improve genetic tips for affected families.

Respiratory symptoms of exposure to workplace substances among Bulgarian dentists

Objectives: Dentists are exposed to a variety of airborne chemicals that can serve as irritants and sensitizers and can give rise to work-related respiratory symptoms. The objective of this study was to estimate the prevalence of respiratory symptoms of exposure to workplace substances and risk factors involved in Bulgarian dentists.

Methods: A cross-sectional study was conducted among Bulgarian dentists using a self-recall questionnaire. A direct acyclic chart (DAG) has been developed to illustrate direct and indirect causal paths between exposure to irritants and / or allergens from the dental environment and work-related respiratory symptoms in dentists. A multiple logistic regression analysis was performed to study the relationship between sex, work experience, daily exposure to chemicals from the dental environment, a history of atopic disorders and respiratory symptoms related to the job.

Results: A total of 4675 dentists completed the questionnaire (response rate 48.1%). The prevalence of self-reported respiratory symptoms was 20.7%. The most commonly repeated causes of respiratory reactions were disinfectants (65.7%) and materials based on acrylic resins (29.7%). Factors associated with work-related respiratory symptoms are a personal history of confidence interval of asthma, work experience> 20 years and female sex.

 

Epoxy Magnetic Particles
EM-20-100	Spherotech	100 mL	EUR 1716
Description: Epoxy Magnetic Particles are prepared by coating a layer of magnetite and polystyrene onto monodispersed (i.e.. uniform sized) polystyrene core particles. The magnetic particles can be easily separated from a suspension magnetically. These particles become non magnetic when removed from a magnet, and do not retain any detectable magnetism even after repeated exposure to strong magnetic field.Are of research that those particles had been tested are cell separation, affinity purification, DNA probe assays, magnetic particle EIA. For more infomation please don't hesiate to contact our technical team.

DURAN Over-Cap 45mm Black Phenolic
BOT2596	Scientific Laboratory Supplies	PK10	EUR 25.08

Phenolic GPI 15-415 Screw Cap
TIS1558	Scientific Laboratory Supplies	PK288	EUR 306.66

c3030 kit epoxy ph
C3030PE	Consort	ea	EUR 1239.6

c6010 kit epoxy ec
C6010KE	Consort	ea	EUR 812.4

c6010 kit epoxy ph
C6010PE	Consort	ea	EUR 795.6

c6030 kit epoxy ph
C6030PE	Consort	ea	EUR 1080

c3010 kit epoxy ph
C3010PE	Consort	ea	EUR 895.2

MagSi-S Epoxy 1.0
MD01010	AMSBIO	2 mL	EUR 139.2

MagSi-S Epoxy 1.0
MD03010	AMSBIO	10 mL	EUR 483.6

MagSi-S Epoxy 1.0
MD04010	AMSBIO	100 mL	EUR 2898

MagSi-S Epoxy 600
MD16010	AMSBIO	2 mL	EUR 153.6

MagSi-S Epoxy 600
MD18010	AMSBIO	10 mL	EUR 572.4

MagSi-S Epoxy 600
MD19010	AMSBIO	100 mL	EUR 3438

MagSi-S Epoxy 3.0
MD41010	AMSBIO	2 mL	EUR 139.2

MagSi-S Epoxy 3.0
MD43010	AMSBIO	10 mL	EUR 483.6

MagSi-S Epoxy 3.0
MD44010	AMSBIO	100 mL	EUR 2898

NuGel™ Poly-Epoxy
NPEY-25	Biotech Support Group	25 Grams	EUR 559.2
Description: NuGel™ Immobilization Reagent

Bottle carrier epoxy small
BGS040	Scientific Laboratory Supplies	EACH	EUR 105.64

MBHA resin
20-abx095143	Abbexa	EUR 594.00 EUR 2766.00 EUR 1062.00 EUR 292.80 EUR 1796.40	100 g 1 kg 250 g 25 g 500 g

Merrifield resin
20-abx095147	Abbexa	EUR 260.40 EUR 427.20 EUR 627.60	100 g 250 g 500 g

Wang resin
20-abx095148	Abbexa	EUR 376.80 EUR 627.60 EUR 1062.00	100 g 250 g 500 g

AM resin
20-abx095149	Abbexa	EUR 376.80 EUR 710.40 EUR 1062.00	100 g 250 g 500 g

Wang Resin
A6776-100000	ApexBio	100 g	EUR 240
Description: Wang Resin

Wang Resin
A6776-25000	ApexBio	25 g	EUR 150
Description: Wang Resin

75cm U-Shaped Phenolic Cap TC Flask
430725U	Scientific Laboratory Supplies	PK100	EUR 290.7

92 L Cooled Incubator epoxy
INC2156	Scientific Laboratory Supplies	EACH	EUR 3954.66

Epoxy-activated Sepharose 6B 15g
17048001	Scientific Laboratory Supplies	EACH	EUR 660.06

GST-Sefinose Resin
BSP031	Bio Basic	5ml	EUR 174.84

GST-Sefinose Resin
BSP031-1	Bio Basic	25ml	EUR 498.48

Glutathione Agarose Resin
4B-GLU-10	ABTBeads	10 ml	EUR 207.6

Glutathione Agarose Resin
4B-GLU-100	ABTBeads	100 ml	EUR 1195.2

Biotin Agarose Resin
4BCL-BI-10	ABTBeads	10 ml	EUR 493.2

Biotin Agarose Resin
4BCL-BI-5	ABTBeads	5 ml	EUR 282

Aminomethyl Polystyrene Resin
5-08191	CHI Scientific	25g	Ask for price

Aminomethyl Polystyrene Resin
5-08192	CHI Scientific	100g	Ask for price

Aminomethyl Polystyrene Resin
5-08193	CHI Scientific	25g	Ask for price

Aminomethyl Polystyrene Resin
5-08194	CHI Scientific	100g	Ask for price

MP-EDC resin
20-abx095129	Abbexa	EUR 1262.40 EUR 526.80 EUR 260.40	100 g 25 g 5 g

MP-TEMPO resin
20-abx095130	Abbexa	EUR 1262.40 EUR 526.80 EUR 260.40	100 g 25 g 5 g

MP-Aminomethyl resin
20-abx095132	Abbexa	EUR 393.60 EUR 226.80	100 g 25 g

MP-Acetylacetone resin
20-abx095133	Abbexa	EUR 526.80 EUR 276.00	100 g 25 g

MP-Piperazinomethyl resin
20-abx095134	Abbexa	EUR 526.80 EUR 276.00	100 g 25 g

MP-Diethylenetriamine resin
20-abx095136	Abbexa	EUR 543.60 EUR 276.00	100 g 25 g

MP-Carbonate resin
20-abx095138	Abbexa	EUR 560.40 EUR 292.80	100 g 25 g

MP-Morpholinomethyl resin
20-abx095139	Abbexa	EUR 560.40 EUR 292.80	100 g 25 g

MP-Thiosulfate resin
20-abx095140	Abbexa	EUR 560.40 EUR 292.80	100 g 25 g

MP-Mercaptomethyl resin
abx095141-100g	Abbexa	100 g	EUR 610.8

MP-TMT resin
20-abx095142	Abbexa	EUR 693.60 EUR 326.40	100 g 25 g

Ni-NTA Resin
20-abx098108	Abbexa	EUR 610.80 EUR 292.80	25 ml 5 ml

Ni-IDA Resin
20-abx098109	Abbexa	EUR 543.60 EUR 276.00	25 ml 5 ml

Protein A Resin
abx098111-5ml	Abbexa	5 ml	EUR 477.6

Protein G Resin
abx098112-5ml	Abbexa	5 ml	EUR 526.8

Rink Amide Resin
A6576-100000	ApexBio	100 g	EUR 1136.4
Description: Rink resin is originally developed for SPS of peptide amides. Now the scope of its application is extended from carboxylic amides to the immobilization of amines, substituted amides.

Rink Amide Resin
A6576-25000	ApexBio	25 g	EUR 358.8
Description: Rink resin is originally developed for SPS of peptide amides. Now the scope of its application is extended from carboxylic amides to the immobilization of amines, substituted amides.

DHP HM Resin
A6789-100000	ApexBio	100 g	EUR 616.8
Description: DHP HM Resin

DHP HM Resin
A6789-25000	ApexBio	25 g	EUR 243.6
Description: DHP HM Resin

DiagNano Epoxy Silica Particles, 300 nm
DNG-F062	Creative Diagnostics	100 mg	EUR 682.8

DiagNano Epoxy Silica Particles, 500 nm
DNG-F063	Creative Diagnostics	100 mg	EUR 682.8

DiagNano Epoxy Silica Particles, 1 μm
DNG-F064	Creative Diagnostics	100 mg	EUR 702

DiagNano Epoxy Silica Particles, 1.5 μm
DNG-F065	Creative Diagnostics	100 mg	EUR 721.2

DiagNano Epoxy Silica Particles, 3 μm
DNG-F066	Creative Diagnostics	100 mg	EUR 682.8

DiagNano Epoxy Silica Particles, 4 μm
DNG-F067	Creative Diagnostics	100 mg	EUR 682.8

DiagNano Epoxy Silica Particles, 5 μm
DNG-F068	Creative Diagnostics	100 mg	EUR 682.8

DiagNano Epoxy Silica Particles, 10 μm
DNG-F069	Creative Diagnostics	100 mg	EUR 721.2

DiagNano Epoxy Silica Particles, 15 μm
DNG-F070	Creative Diagnostics	100 mg	EUR 721.2

DiagNano Epoxy Silica Particles, 20 μm
DNG-F071	Creative Diagnostics	100 mg	EUR 721.2

DiagPoly Epoxy Polystyrene Particles, 30 μm
DNM-M001	Creative Diagnostics	10 mL	EUR 925.2

SLS Electrode pH Epoxy Body BNC
PHE1000	Scientific Laboratory Supplies	EACH	EUR 62.7

Combination pH electrode with epoxy body
9156BNWP	Scientific Laboratory Supplies	EACH	EUR 214.32

Black Phenolic screw thread cap open top linerless autoclavable
VIA2522	Scientific Laboratory Supplies	PK144	EUR 49.93

Protein A Agarose Resin
BSP095-7	Bio Basic	1ml	EUR 112.2

Protein A Agarose Resin
BSP095-8	Bio Basic	5ml	EUR 294.9

Protein A Sefinose Resin
BSP096-25	Bio Basic	12.5ml	EUR 843

Protein A Sefinose Resin
BSP096-5	Bio Basic	2.5ml	EUR 232.26

Nickel NTA Agarose Resin
6BCL-NTANi-100	ABTBeads	100 ml	EUR 781.2

Nickel NTA Agarose Resin
6BCL-NTANi-25	ABTBeads	25 ml	EUR 260.4

Nickel NTA Agarose Resin
6BCL-NTANi-500	ABTBeads	500 ml	EUR 3100.8

Protein L Agarose Resin
4BCLPL-10	ABTBeads	10 ml	EUR 471.6

Protein L Agarose Resin
4BCLPL-2	ABTBeads	2 ml	EUR 236.4

Protein L Agarose Resin
4BCLPL-5	ABTBeads	5 ml	EUR 340.8

Fmoc-Ala-Wang resin
5-07835	CHI Scientific	5g	Ask for price

Fmoc-Ala-Wang resin
5-07836	CHI Scientific	25g	Ask for price

Fmoc-Acp-Wang resin
5-07841	CHI Scientific	5g	Ask for price

Fmoc-Acp-Wang resin
5-07842	CHI Scientific	25g	Ask for price

Fmoc-Cha-Wang Resin
5-07863	CHI Scientific	5g	Ask for price

Fmoc-Cha-Wang Resin
5-07864	CHI Scientific	25g	Ask for price

Fmoc-Cit-Wang resin
5-07867	CHI Scientific	5g	Ask for price

Fmoc-Cit-Wang resin
5-07868	CHI Scientific	25g	Ask for price

Fmoc-Gly-Wang resin
5-07891	CHI Scientific	5g	Ask for price

Fmoc-Gly-Wang resin
5-07892	CHI Scientific	25g	Ask for price

Fmoc-Gly-Wang resin
5-07893	CHI Scientific	5g	Ask for price

Fmoc-Gly-Wang resin
5-07894	CHI Scientific	25g	Ask for price

Fmoc-Ile-Wang resin
5-07899	CHI Scientific	5g	Ask for price

Fmoc-Ile-Wang resin
5-07900	CHI Scientific	25g	Ask for price

Fmoc-Leu-Wang resin
5-07903	CHI Scientific	5g	Ask for price

Fmoc-Leu-Wang resin
5-07904	CHI Scientific	25g	Ask for price

Fmoc-Leu-Wang resin
5-07905	CHI Scientific	5g	Ask for price

Fmoc-Leu-Wang resin
5-07906	CHI Scientific	25g	Ask for price

Fmoc-Lys-Wang resin
5-07907	CHI Scientific	5g	Ask for price

Fmoc-Lys-Wang resin
5-07908	CHI Scientific	25g	Ask for price

Fmoc-Met-Wang resin
5-07923	CHI Scientific	5g	Ask for price

Fmoc-Met-Wang resin
5-07924	CHI Scientific	25g	Ask for price

Fmoc-Phe-Wang resin
5-07929	CHI Scientific	5g	Ask for price

Fmoc-Phe-Wang resin
5-07930	CHI Scientific	25g	Ask for price

Fmoc-Phe-Wang resin
5-07931	CHI Scientific	5g	Ask for price

Fmoc-Phe-Wang resin
5-07932	CHI Scientific	25g	Ask for price

Fmoc-Pro-Wang resin
5-07947	CHI Scientific	5g	Ask for price

Fmoc-Pro-Wang resin
5-07948	CHI Scientific	25g	Ask for price

Fmoc-Pro-Wang resin
5-07949	CHI Scientific	5g	Ask for price

Fmoc-Pro-Wang resin
5-07950	CHI Scientific	25g	Ask for price

Fmoc-Tle-Wang Resin
5-07965	CHI Scientific	5g	Ask for price

Fmoc-Tle-Wang Resin
5-07966	CHI Scientific	25g	Ask for price

Fmoc-Thr-Wang resin
5-07967	CHI Scientific	5g	Ask for price

Fmoc-Thr-Wang resin
5-07968	CHI Scientific	25g	Ask for price

Fmoc-Trp-Wang resin
5-07975	CHI Scientific	5g	Ask for price

Fmoc-Trp-Wang resin
5-07976	CHI Scientific	25g	Ask for price

Fmoc-Val-Wang resin
5-07989	CHI Scientific	5g	Ask for price

Fmoc-Val-Wang resin
5-07990	CHI Scientific	25g	Ask for price

2-Chlorotrityl Chloride Resin
5-08183	CHI Scientific	25g	Ask for price

2-Chlorotrityl Chloride Resin
5-08184	CHI Scientific	100g	Ask for price

2-Chlorotrityl Chloride Resin
5-08185	CHI Scientific	25g	Ask for price

2-Chlorotrityl Chloride Resin
5-08186	CHI Scientific	100g	Ask for price

Fmoc-Leu-Tcp Resin
5-08289	CHI Scientific	5g	Ask for price

Fmoc-Leu-Tcp Resin
5-08290	CHI Scientific	25g	Ask for price

Protein A-Agarose Resin
55000	AAT Bioquest	1 mL	EUR 158.4

Protein A-Agarose Resin
55005	AAT Bioquest	5 mL	EUR 367.2

Fmoc-Gly-Wang resin
20-abx095122	Abbexa	EUR 661.20 EUR 309.60	100 g 25 g

MP-Sulfonic acid resin
20-abx095135	Abbexa	EUR 526.80 EUR 276.00	100 g 25 g

2-Chlorotrityl Chloride resin
20-abx095137	Abbexa	EUR 560.40 EUR 1078.80 EUR 276.00 EUR 1930.80	100 g 250 g 25 g 500 g

Rink Amide AM resin
20-abx095146	Abbexa	EUR 961.20 EUR 4987.20 EUR 1746.00 EUR 393.60 EUR 2698.80	100 g 1 kg 250 g 25 g 500 g

Rink Amide-AM Resin
A6780-100000	ApexBio	100 g	EUR 872.4
Description: Rink Amide-AM Resin

Rink Amide-AM Resin
A6780-25000	ApexBio	25 g	EUR 466.8
Description: Rink Amide-AM Resin

Rink Amide-AM Resin
A6780-5000	ApexBio	5 g	EUR 262.8
Description: Rink Amide-AM Resin

2-Chlorotrityl Chloride Resin
A6791-100000	ApexBio	100 g	EUR 393.6
Description: 2-Chlorotrityl Chloride Resin

2-Chlorotrityl Chloride Resin
A6791-25000	ApexBio	25 g	EUR 170.4
Description: 2-Chlorotrityl Chloride Resin

Fmoc-Phe-Wang resin
A6831-25000	ApexBio	25 g	EUR 246
Description: Fmoc-Phe-Wang resin

Fmoc-Phe-Wang resin
A6831-5000	ApexBio	5 g	EUR 104.4
Description: Fmoc-Phe-Wang resin

Fmoc-Ile-Wang resin
A6836-25000	ApexBio	25 g	EUR 246
Description: Fmoc-Ile-Wang resin

Fmoc-Ile-Wang resin
A6836-5000	ApexBio	5 g	EUR 104.4
Description: Fmoc-Ile-Wang resin

Fmoc-Gly-Wang resin
A6839-25000	ApexBio	25 g	EUR 246
Description: Fmoc-Gly-Wang resin

Fmoc-Gly-Wang resin
A6839-5000	ApexBio	5 g	EUR 104.4
Description: Fmoc-Gly-Wang resin

Protein A Agarose Resin
PA09-100	ABTBeads	100 ml	EUR 2512.8

Protein A Agarose Resin
PA09-25	ABTBeads	25 ml	EUR 1110

Protein A Agarose Resin
PA09-5	ABTBeads	5 ml	EUR 349.2

Protein G Agarose Resin
SF022-PG	Bio Basic	5ml	EUR 930

Streptavidin 6HC Agarose Resin
STV6HC-10	ABTBeads	10 ml	EUR 478.8

Conclusion: Work-related respiratory symptoms are common among dentists and indicate efforts to establish effective programs and techniques for reducing or eliminating direct exposure to chemicals in the air in the dental environment.