Recent research indicated that exposure to residential vegetation (“greenery”) may be protective against cognitive decline and can support the integrity of the corresponding brain structures. However, it is still unclear about these effects, especially in less rich and middle-aged countries. In this study, we investigated the associations between greenery and neurocognitive function. We used a convenience sample of 112 middle-aged Bulgarians and two cognitive tests: the consortium for establishing a register for the neuropsychological battery of Alzheimer’s disease (CERAD-NB) and Cognitive Assessment of Montreal (MOCA). In addition, structural cerebral imaging data was available for 25 participants.
The address of the home of the participants was used to connect the cognition scores to the normalized difference vegetation index (NDVI), a measurement of the level of global vegetation of neighborhood (radii from 100 to 1000 m). The results indicated that NDVI higher was always associated with higher scores of CERAD-NB and MOCA through radial buffers and adjustment scenarios. The lower waist tower media the effect of NDVI on Cerad-NB. NDVI100-M has been positively associated with an average cortical thickness on both hemispheres, but these correlations have been marginally significant (p <0.1) after correction of the false discovery rate due to several comparisons. In conclusion, life in a greener neighborhood could be associated with better cognitive function in middle-aged Bulgarians, with central adipososity lower than partial accounting for this purpose. Provisional evidence suggests that greenery could also contribute to structural integrity in brain regions governing cognitive functions. Future research should rely on our conclusions and investigate larger and more representative population groups.
Criteria system for the treatment of acromegaly treatment in Bulgaria
Acromegaly is a rare endocrine disorder caused by excessive and long-time secretion of growth hormone (GH) by somatotrophasse and resulting from this overproduction of an insulin-type growth factor – 1 hormone (IGF-1) by the liver. There are few registers and clinical data quite limited on the treatment of acromegaly. The analysis of acromegaly data is rarely subject to a system of criteria for assessing the treatment of acromegaly. The novelty of this document is that it presents a case study of real practice on the results of the implementation of such a system in Bulgaria. Analysis of the case study illustrates a clinical information approach to handle thousands of records in the Bulgarian acromegaly database. The results below indicated evidence of the difficulties encountered in maintaining GH and IGF-1 levels in their reference values in the treatment of acromegaly.
The current research extends the results of the evaluation by allowing semantic interoperability between acromegaly databases based on the specification of the OPENEHR. The addition of the IA 2-AB as a second immunological marker identified 94.4%, while the use of ZNT8-AB in second place identified 98.8% of cases. 24.4% presented positive thyroid antibodies and 33.8% had AIT. No relationship was found between the island and AIT antibodies. None of the patients have been positive TTG-IGA-AB. No significant correlation has been established between antibodies with different organ specificities. The purpose of the study was to evaluate the prevalence and relationship of the most frequent autoimmune diseases and self-antibodies of the autoimmune thyroid disease (AIT) and coeliac disease in Adult Bulgarian patients with short-term type 1 diabetes.
A feasibility study on the association between residential greenness and neurocognitive function in middle-aged Bulgarians
Validation of Levenson’s self-signing psychopathic scale to people dependent on the Bulgarian substance
The co-occurrence of disorders of psychopathy and substance (SADS) is associated with higher relapse rates and at an increased risk of violent criminalization. Studies on the validity of psychopathic measures in community samples and dependent people of substances (SDIS) are rare. The current study was to examine the psychometric properties of Levenson’s self-assessment psychopathy scale (LSRP) in a sample of Bulgarian SDIS and non-dependent orders. We have tested 615 participants: 106 heroin users, 91 Amphetamine users, 123 users of polysubstance and 295 commands.
The confirmation factor analyzes replicated the Tri-Factor structure of the SRPA (Egocentric, Antisocial, Calleux). The scale has demonstrated acceptable reliability and validity. SDIS has increased considerably than total controls and sub-ladders of the PSPA, indicating a good discriminant validity. Overall, the results indicate that the SRPA is a valid instrument for measuring psychopathy in the samples of the Bulgarian community. In line with the efforts to improve food practices, this comparative study assessed the objective comprehension of five different front package labels (reference inputs, multiple traffic lights, warning label, nutri-score and health Star) A sample of 1010 Bulgarian adults.
Description: A Calcein AM efflux assay to screen a wide range of compounds for multidrug resistance transporter activityExcitation: 528 nm | Emission: 617 nmCalcein AM is a membrane-permeant, non-fluorescent dye that enters cells passively. Once inside the cytosol of cells, intracellular esterases convert it to fluorescent Calcein (Ex/Em: 495 nm/515 nm), resulting in uniform cytosolic fluorescence. Drug efflux transporters, such as P-glycoprotein (Pgp, MDR1) and multidrug-resistance-associated protein (MRP1), actively extrude Calcein AM from inside the cell before esterases can convert it to Calcein. The presence of additional MDR substrates or inhibitors of MDR expression results in decreased Calcein AM efflux, causing a measurable increase in intracellular fluorescence. In addition to identifying MDR substrates and inhibitors, this kit can also be used to evaluate the activity of MDR transporters in cells.When following our protocol, ION’s MDR Activity kit provides enough reagents to make 100 mL of working solution, enough for ten 96- or 384-well plates or 80 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.
Plasmid Extraction Maxi Plus Kit (10prep), Ion Exchange. (up to 2.2 mg plasmid)
Description: No wash, ratiometric calcium flux assay, 10 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nMRatiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
ION Essentials Ratiometric Calcium - No wash ratiometric calcium assay kit
Description: No wash, ratiometric calcium flux assay, 2 plates. Total assay solution compatible with plate reader and fluorescence microscopy applications. Excitation: 340, 380 nm | Emission: 505 nm | Kd: 145 nMRatiometric Calcium Essentials kit provides the necessary reagents for conducting no wash, ratiometric calcium flux assays compatible with plate reader and fluorescence microscopy applications. Individual components are provided to give the user the flexibility needed to customize your assay.
ION Vital - MitoVolt, mitochondrial membrane potential assay kit with JC-10
Description: Mitochondrial membrane potential assay kit that uses JC-10. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays.Healthy: Ex 540 nm / Em 590 nm | Compromised: Ex 490 nm / Em 525 nmION Vital - MitoVolt assay kit is the ideal solution for detecting changes in mitochondrial membrane potential due to cell apoptosis or other stress-inducing phenomena. MitoVolt can be used to monitor and/or visualize mitochondrial membrane potential as a static endpoint or in real-time. When following our protocol, the MitoVolt kit provides enough reagents to make 25 mL of working solution, enough for five 96-well plates or 500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations and assay volumes for your application.JC-10 is a mitochondrial membrane potential probe. It possesses superior aqueous solubility compared to its better known analogue, JC-1. At low concentrations, JC-10 is monomeric and emits a green fluorescence. JC-10 accumulates in healthy mitochondria, forming J-aggregates that exhibit an orange fluorescence. Mitochondrial depolarization, a key marker of cellular apoptosis, results in a loss of JC-10 accumulation and a reversal to its monomeric form. This reversible behavior of JC-10 allows for ratiometric analysis of mitochondrial membrane potential, where a shift from orange (Ex/Em: 540nm/590nm) to green fluorescence (Ex/Em: 490nm/525nm) is indicative of compromised mitochondria.
ION Vital - Viability Kit, live or dead assay kit for mammalian cells
Description: A rapid and simple two-color assay to discriminate between live and dead cells. Compatible with fluorescence microscopy, flow cytometry, and plate reader assays.Live: Ex 495 nm / Em 515 nm | Dead: Ex 528 nm / Em 617 nmION Vital - Viability kit can be used to quantify live and dead cells within eukaryotic cell suspensions or adherent cultures, 3D cultures, organoids, and some non-fixed tissues. Cannot be used for yeast or bacteria. When following our protocol, the Viability kit provides enough reagents to make 150 mL of working solution, enough for fifteen 96-well plates or 1,500 flow cytometry samples. The actual number of assays will vary according to optimal dye concentrations for your application.Calcein AM is a membrane-permeant, non-fluorescent form of calcein that enters cells passively. Once inside the cytosol of cells, Calcein AM is converted to green fluorescent Calcein by ubiquitous esterases in viable cells, resulting in uniform cytosolic fluorescence (Ex/Em 495 nm/515 nm). Calcein, a polyanionic dye, is membrane-impermeant and is well retained within the cytosol of most healthy cells with intact cell membranes. Ethidium homodimer I (EthD-I) is a membrane-impermeable, high-affinity, nucleic acid stain that is excluded by viable cells with intact cell membranes. When membrane integrity is compromised, a hallmark of dead or dying cells, EthD-I enters the cell and binds DNA, which results in a >30-fold enhancement in bright red, nuclear fluorescence (Ex/Em 528 nm/617 nm).
The objective understanding has been evaluated by comparing the results of two nutrition quality classification tasks (with and without FOPL) in a randomized online experience presenting three food categories (pizza, cakes, breakfast cereals). Multivarioocial ordinal logistic regression models in food categories and through foods were fit. Nutri-score, which is a summary, interpretative, polychromatic FOPL, appeared as the most effective model in the Bulgarian context, with the potential to help consumers better understand the nutritional quality of food. The conclusions are of particular interest to public health decision-makers in the region and throughout Europe, the debate on a FOPL model at EU-wide level continues to gather momentum.
